+1(415) 260-6514 |Jacqueline_Howells@brown.edu| www.jacquelinehowells.com
Brown University, Providence, RI
Doctor of Philosophy: Aug 2017 - Present
Principal Investigators: Dr. Marc Tatar & Dr. Karla Kaun
Department: Ecology and Evolutionary Biology & Neuroscience
Relevant Coursework: The Biology of Aging, Neurogenetics and Disease, Advanced Neurobiology, Molecular Mechanisms of Disease, Innate Immunology, Host-Microbe Interaction, The Diseased Brain.
University of California, Berkeley, CA
Bachelor of Science, Aug 2009 - May 2013
Major: Environmental Science – Emphasis in Biology
Minor: Italian Studies
Relevant Coursework: Senior Research Seminar, Integrative Biology Field Studies, Wildlife Ecology, Evolution, Evolutionary Genetics, Methods of Environmental Science, Organic Chemistry, Global Environmental Problems, American Wildlife, General Biology, General Chemistry, Cognitive Science Group Study.
Senior Thesis: Microhabitat use of the Eastern fence lizard (Sceloporus undulatus) on the White Sands ecotone.
Lowell High School, San Francisco, CA
High School Diploma, Aug 2005 - Jun 2009 Graduated with Honors
Brown University , Providence, RI - Ecology & Evolutionary Biology Department, Neuroscience Department
Principle Investigators: Dr. Marc Tatar & Dr. Karla Kaun
PhD Student, Pathobiology Program, Sept 2017 – Present
Research Focus: Investigating the mechanisms underlying the pathogenesis of neurodegenerative disease and associated behavioral abnormalities using transgenic drosophila models of Alzheimer’s Disease, Tauopathies, and Huntington’s Disease .
University of California, San Francisco, CA - Diabetes Center
Principle Investigator: Dr. Jeffrey Bluestone
Staff Research Associate, Sept 2013 – June 2017
Advisor: Dr. Hilary Thomas Research Focus I: Investigating cellular phenotype and function associated with markers of βeta cell function in patients with longstanding Type I Diabetes
Assisted in scheduling and performing blood draws, processing PBMCs.
Developed and refined human immunological assays and culture techniques including sorting T Regulatory Cells and T Effector Cells to perform functional assays.
Performing flow cytometer analysis, developed antibody-staining panels.
Analyzed using FlowJo, BD FACS DIVA, and PRISM.
Trained summer medical student in flow cytometry.
Advisor: Dr. Eleonora Trotta Research Focus II: Investigating the mechanism of action and function of modified LFA3-Fc molecules in suppressing activated T cells.
Assist industry collaborators with in vitro assays to screen candidate molecules.
Assess in vivo activity of molecules using a humanized mice and human PBMCs
Advisor: Dr. Caroline Raffin Research Focus III: Development of citrullinated-vimentin-specific CAR targeting Tregs to treat autoimmune rheumatoid arthritis.
Assisted in bacterial transfection with CV Specific CAR.
Performed PCR and gel electrophoresis to confirm transfection
Performed immunohistochemical staining on osteoclast cells
University of California, Berkeley, CA- Dept of Environmental Science Principal Investigator: Dr. Erica Rosenblum
Undergraduate Researcher/Research Associate, May 2012–Sep. 2013
Advisor: Dr. Simone Des Roches Research Focus I: Microhabitat use of the Eastern fence lizard (Sceloporus undulatus) on the White Sands ecotone.
Collected data on Eastern Fence Lizard species morphology, phenotype, and microhabitat.
Performed Statistical analysis with R software.
Composed thesis and presented thesis.
Research Focus II: Assisted graduate student Dr. Simone Des Roches in her Ph.D. project on ongoing natural selection and rapid adaptation of lizard species in White Sands.
Collected lizards in multiple desert sites by noosing.
Assisted in developing a less invasive protocol for collecting DNA samples from lizard saliva using buccal swabs
Analyzed samples to determine genotype at the melanocortin-1 receptor (Mc1r) using PCR and Gel electrophoresis.
Aided in new project regarding species of crickets in White Sands.
University of California, Berkeley, Dept of Molecular and Cellular Biology Principle Investigator: Dr. David Raulet
Laboratory Assistant, Mar 2011- Sep 2013
Determine phenotype the phenotype and genotype of RAG OT1 transgenic and B6 NKG2D mice and for lab experimental use using flow cytometry and PCR
Assisted in weaning and breeding mice.
Trained and scheduled incoming undergraduate laboratory assistants.
Assisted the lab manager with administrative duties including ordering and inventory.
Outreach and Services
Fly Club, Brown University
Co-Coordinator, November 2018-Present
Purpose: To coordinate meeting for drosophila researchers at Brown once a month to share drosophila research ideas and techniques as well as foster a larger drosophila community by inviting outside speakers to present drosophila related research.
Pathobiology Peer Mentorship, Brown University
Peer Mentor, August 2018-Present
Purpose: To provide guidance to incoming Pathobiology students.
Society of Women in Science, University of California, Berkeley
Activities Planner and Historian, May 2011-May 2013
Purpose: Providing educational support and opportunities targeted towards women and girls interested in STEM fields
Women in Science Speaker Series-Helped host U.C. Berkeley female faculty who shared their experiences in STEM fields to undergraduates
UC Berkeley Relay For Life- held fundraiser provided educational material regarding breast cancer.
UC Berkeley Expanding Your Horizons-volunteered as an undergrad mentor and lead group of girls in 6th-9th grade through different workshops focused on chemistry, biology, physics, and computer science.
Saves the Frogs Day- University of California, Berkeley
Instructor, April 2012
Purpose: Event for elementary school children to increase awareness of declines in populations of amphibians worldwide due to chytrid pathogens.
White Sands Community Camp, University of California, Berkeley
Instructor, May 2012-May 2013
Purpose: To lead 2-day educational experience for children and the community surrounding White Sands on the National Park ecosystem.
Cal WonderWorks, University of California, Berkeley
Volunteer Instructor, Sep 2010- May 2011
Purpose: visit and elementary school bi-monthly to demonstrate interactive science experiments.
Awards and Presentations
National Science Foundation Research Experience for Undergradtes. May 2012 Purpose: Awarded to conduct thesis research in the Rosenblum Lab, UC Berkeley
"Microhabitat Use of the Eastern Fence Lizard on the White Sands Ecotone”, May 2013 Purpose: Presentation given at the Environmental Science Symposium, UC Berkeley.
Sheridan Teaching Certificate I
Cell Culture: Suppression assays, intracellular cytokine staining, dose-response titrations, mixed lymphocyte reaction
Flow Cytometry: Experienced using the Fortessa, Aria Cell Sorter, FACS Calibur, and LSRII
Mouse splenectomy, intravenous tail injection and intraperitoneal injection.
HIPAA trained and certified
Dr. Marc Tatar -Marc_Tatar@brown.edu
Dr. Karla Kaun- Karla_Kaun@brown.du
Dr. Jeffrey Bluestone - firstname.lastname@example.org
Dr. Hilary Thomas - email@example.com
Dr. Eleonora Trotta - firstname.lastname@example.org
Dr. Caroline Raffin - email@example.com
Dr. Erica Bree Rosenblum - firstname.lastname@example.org
Dr. Simone Des Roches - email@example.com